arlette5466

Background was washed out with deionized water and gel was scanned with the default instrument settings for analysis (Lexmark CX727 scanner). Inhibition of glycolipid synthesis was carried out as described previously (36) with some modifications. Resialylation was carried out with 50 mU/ml sialyltransferase in α-MEM for 2 h at 37°C. In the control groups, Lec-2 […]

In addition, we determined by inhibitor (N-benzyl GalNAc)- and cell line-specific (Lec-1) studies that AAV1 and AAV6 require N-linked and not O-linked sialic acid. E. coli can transport and catabolize the common sialic acid, Neu5Ac, as a sole source of carbon and nitrogen but also related sialic acids, N-glycolylneuraminic acid (Neu5Gc) and 3-keto-3-deoxy-d-glycero-d-galactonononic acid (KDN),